In 2004, a Mario game had a transgender playable character with a subplot about being accepted by her family. This was censored in the American version.
Vivian is a major character in Paper Mario: The Thousand-Year Door. Vivian originally worked as a member of the villainous Shadow Sirens alongside her sisters, Beldam and Marilyn. Later, Vivian defects from the Shadow Sirens and joins Mario as his fifth party member. She is consistently put down by her oldest sister, Beldam, and has had a poor relationship with both her sisters for quite some time when Mario meets her. Although she suffers from an inferiority complex throughout the events of the game, she learns to respect herself as she travels with Mario. In the original Japanese version of the game and some translations, Vivian is depicted as transgender, identifying as female despite other characters asserting that she is male, but this is lost in the English version and she is only described with feminine pronouns[1].
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